Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background | Gallus gallus | Charles River Laboratories, RRID:SCR_003792 | Specific-pathogen-free chicken (SPF) eggs | |
| Strain, strain background | Gallus gallus | Susan Chapman at Clemson University; South Carolina; USA, RRID:SCR_011159
McGrew et al., 2004 |
Cytoplasmic GFP eggs | |
| Strain, strain background | Northern Bobwhite quail | Ozark Hatcheries Bénazéraf et al., 2017 |
Transgenic quails expressing H2B-Cherry | |
| Chemical compound, drug | Paraformaldehyde | Sigma | 158127 | |
| Antibody | T/Brachyury (goat polyclonal) | R&D Systems | AF2085 | IF (1/1000) |
| Antibody | SOX2 (rabbit polyclonal) | Millipore RRID:AB_2286686 | Cat# AB5603 | IF (1/1000) |
| Antibody | E-Cadherin (mouse monoclonal) | Abcam RRID:AB_1310159 |
ab76055 | IF (1/250) |
| Antibody | N-Cadherin (rabbit polyclonal) | Abcam RRID:AB_298943 |
ab12221 | IF (1/250) |
| Antibody | Fibronectin (mouse) | DSHB | MT4S | IF (1/50) |
| Antibody | Laminin (rabbit) | Sigma-Aldrich RRID:AB_477163 | Cat# L9393 | IF (1/200) |
| Antibody | Phospho-histone 3 (rabbit polyclonal) | Santa Cruz BiotechnologyRRID:AB_2233067 | sc-8656 | IF (1/1000) |
| Antibody | MSGN1 (rabbit polyclonal) | Pourquie Laboratory Oginuma et al., 2017 |
IF (1/1000) | |
| Recombinant DNA reagent | pCAGG-H2B- Venus | Pourquie Laboratory Denans et al., 2015 |
pCAGG backbone | |
| Recombinant DNA reagent | pCAGG-H2B-RFP | Pourquie Laboratory Denans et al., 2015 |
pCAGG backbone | |
| Recombinant DNA reagent | pCAGG-GAP43-Venus | Pourquie Laboratory Oginuma et al., 2017 |
pCAGG backbone | |
| Chemical compound, drug | NucRed Live 647 ReadyProbes Reagent | Thermo Fisher | R37106 | Two drops in 1 ml |
| Software, algorithm | MOSAIC plug-in from ImageJ | ImageJ Sbalzarini and Koumoutsakos, 2005 |
||
| Software, algorithm | Tracs reconstruction | Arthur Michaut | This study | Python-based homemade code |
| Software, algorithm | K mean Brainbow clustering | Figure 2—source data 2 | MATLAB | |
| Recombinant DNA reagent | pQCGICIDPA | Retroviral barcoding | This study | pQCXIX backbone |
| Recombinant DNA reagent | Tol2-CAG::Nucbow | RRID:Addgene_158992
Loulier et al., 2014 |
pCX backbone |