Skip to main content
. 2021 Jul 6;10:e64932. doi: 10.7554/eLife.64932

Figure 4. Androgen-sensitive differentially methylated probes (asDMPs) in sheep ear.

(A) MKLN1 (cg21524116, p=1.05E−27), (B) ETAA1 (cg01822430, p=1.31E−13), (C) LMO4 (cg15851301, p=1.62E−09), and (D) KIAA2026 (cg00658920, p=2.46E−09). The p-values were calculated using a t-test of the difference in linear regression slopes.

Figure 4.

Figure 4—figure supplement 1. Androgen-sensitive differentially methylated probes (asDMPs).

Figure 4—figure supplement 1.

(A) Chromosome location of all statistically significant asDMPs in all sheep groups. Y-axis shows the proportion of probes that map to each chromosome, normalized for chromosome size and percentage of significant probes within each comparison group. (B) All 4694 statistically significant (p<0.05) asDMPs ordered by p-value. (C, D) Observed overexpected (O/E) ratios for (C) androgen receptor (AR) and (D) the glucocorticoid receptor (NR3C1) binding to windows of 50 asDMPs, calculated from the Cistrome dataset (Figure 5).
Figure 4—figure supplement 2. Mass and methylation in young male sheep.

Figure 4—figure supplement 2.

(A) Mass (kg) of male lambs (<1 year) according to castration status (castrated males, green; intact males, gray; p=<0.001, error bars = SEM). p-Value was calculated using a standard t-test. (B) A positive relationship exists between castrated young males (green) and methylation at androgen-sensitive differentially methylated probes (asDMPs), but a negative correlation between intact males and methylation (gray). (C) Significance of this difference in association extends for approximately the top 1000 asDMPs. Red line indicates a significance threshold, that is, the 99th quantile for 1000 bootstrap replicates of 100 random probes.