Figure 7.

Citrate also potentiates ASIC lacking ASIC1 subunit in mouse primary sensory neurons. A, Primary sensory neurons isolated from the mouse dorsal root ganglia were perfused in a bath solution, pH 7.4, containing no citrate, and each stimulus consisted of a 3 s exposure (at 30 s intervals) to a test solution, pH 5.5, with or without citrate (25 mm) or amiloride (100 μm). B, Summarized result from A. C, D, Similar to B, except the primary sensory neurons were from mice lacking ASIC1 (ASIC1^−/−; C) and ASIC2 (ASIC2^−/−; D), respectively. In B–D, error bars indicate the mean ± SEM; n = 5 per group, **p < 0.01, ***p < 0.001, one-way ANOVA with a post hoc Tukey's test.