Figure 9.

Acetic acid but not citric acid induces writhing behavior that is ASIC1 independent. A, B, Each mouse received intraperitoneal injection of either 1% acetic acid, pH 3.5; 30 mm citric acid, pH 5.5; or 100 mm citric acid, pH 3.5; and the numbers of abdominal contractions (A) and body contortions (B) over a period of 10 min were recorded; n = 5 per group. n.s. p > 0.05, ***p < 0.001, one-way ANOVA with a post hoc Tukey's test. C, D, Each mouse received intraperitoneal injection of 1% acetic acid, pH 3.5, with or without amiloride (100 μm), and the numbers of abdominal contractions (C) and body contortions (D) over a period of 10 min were recorded; n = 5 per group, n.s. p > 0.05, two-tailed Student's t test. E, F, wild-type mice (WT) or mice with homozygous ASIC1 deletion (ASIC1^−/−) received intraperitoneal injection of 1% acetic acid, pH 3.5, and the numbers of abdominal contractions (E) and body contortions (F) over a period of 10 min were recorded; n = 6 per group, n.s. p > 0.05, two-tailed Student's t test. n.s., Not significant.