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. 2021 Jun 7;595(7866):278–282. doi: 10.1038/s41586-021-03676-z

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Extended Data Fig. 1 — Alignment of the 30 VHH genes, highlighting the 100% amino acid conservation (in red) and the 4 hydrophilic amino acids in framework (FR) 2 (in blue). In VH proteins, these latter four amino acids are hydrophobic and mediate the interaction with light chains. Schematics below show the configuration of VHH gene units, composed of a mouse VH promoter (250 bp, containing the octamer and TATA box); mouse leader exons–intron (about 150 bp) encoding the signal peptide cleaved off during heavy chain processing in the endoplasmic reticulum; the camelid VHH open reading frame (about 300 bp); and mouse downstream sequences (100 bp) containing the recombination signal sequences (RSSs).