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. 2021 Jun 7;595(7866):278–282. doi: 10.1038/s41586-021-03676-z

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Top table shows nanobody and JH use for the 16 VHH clones isolated from immunized nanomice. Middle graph shows protein alignment for VHHs isolated from HIV-1 trimer immunized nanomice. CDRs are boxed. Bottom table shows hypermutation profiles for VHH, D and J domains of selected nanobodies. b, Left, BLI analysis of BG505 DS-SOSIP binding to immobilized VHH9-1. Red trace represents the raw data, and the kinetic fit is shown in grey underneath. Right, table showing the kinetic constants for association (k_on), dissociation (k_off) and equilibrium (K_D) for all four VHH9 nanobody variants.