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. Author manuscript; available in PMC: 2021 Dec 15.
Published in final edited form as: Cancer Res. 2021 Feb 1;81(12):3255–3269. doi: 10.1158/0008-5472.CAN-20-3892

Figure 5. ADAM10 KO and RT reduce growth of metastatic and orthotopically implanted KPC tumors.

Figure 5.

A. Exponential growth rate (k) of human organoid line Panc193, in co-culture with human CAFs, measured by Incucyte live cell analyzer after treatment with 0 or 10Gy RT, in the presence or absence of ADAM10 inhibitor GI254023x. B. Cell viability of human Panc193 organoids, in coculture with human CAFs, 1 week following treatment with 0 or 10Gy RT, in presence or absence of ADAM10 inhibitor GI254023x. C. Cell viability of human Panc193 organoids, in coculture with human CAFs, 1 week following treatment with 0 or 10Gy RT, in presence or absence of EPHB4 inhibitor TNYL-Raw(B4i)). D. Schema of hemispleen metastatic liver tumor model utilized in following slides. E. Representative images of mouse livers collected at sacrifice, 2 weeks following implantation with fibroblast cocultured WT or ADAM10 KO PK5L1940 cells which had been treated with 0 or 10Gy RT 1 week prior, Created with BioRender.com. F. Number of metastatic liver lesions counted at necropsy, 14 days after hemispleen implantation of fibroblast cocultured WT or ADAM10 KO PK5L1940 cells after treatment with 10Gy RT. G. Proportion of liver surface area comprised of tumor at day 14 post implant of WT or ADAM10 knockout PK5L1940 cells after treatment with 10Gy RT. H. Survival in mice treated with hemispleen implantation of metastatic liver tumors, using fibroblast cocultured WT and ADAM10 KO PK5L1940 cells, N=5. P values shown derived from Mantel-Cox log rank analysis. I. Liver mass of mice 14 days after hemispleen implantation with WT PK5L1940 cells co-cultured with WT or EphrinB2 KO NIH 3T3 cells. J. Proportion of liver surface area comprised of tumor 14 days after hemispleen implantation with WT PK5L1940 cells co-cultured with WT or EphrinB2 KO NIH 3T3 cells. K. Tumor volume, 27 days following implantation of WT or ADAM10KO tumors into flanks of PDGFRa-ADAM10 fl mice or littermate controls. L. Tumor volume of WT tumors 27 days following implantation into flanks of PDGFRa-ADAM10 fl mice or littermate controls, all treated with 16Gy RT 7 days following implantation. N>3, error bars represent SEM. P values calculated using student’s T-test. * indicates p<0.05, ** p<0.02, *** indicates p<0.001, **** indicates p<0.0001