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. 2021 May 21;129(2):326–341. doi: 10.1161/CIRCRESAHA.121.318868

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© 2021 The Authors.

Circulation Research is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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Figure 5. — Automated CalTrack quantification of calcium concentration and kinetic parameters in guinea pig wild-type (WT) and TnnI3^R145G/+ cardiomyocytes is comparable to manual IonOptix IonWizard software analysis. A, Average calcium transients are identical by analysis via IonWizard or CalTrack analysis. Average traces are made of n=42 WT and n=36 for TnnI3^R145G/+ cells. B, Calcium amplitude is unaffected by Tnni3^R145G/+. C, Baseline [Ca²⁺] is increased in TnnI3^R145G/+ cells. Time to 10% of peak (T10_on; D), time to 50% of calcium peak (T50_on; E), and time to 90% of peak (T90_on; F) are unaffected by TnnI3^R145G/+. G, time to reach calcium peak (T_on) is significantly longer in TnnI3^R145G/+ cardiomyocytes when assessed by CalTrack analysis. H, Time of 10% decay (T10_off) is unaffected by TnnI3^R145G/+. I, Time of 50% calcium decay (T50_off) is longer in TnnI3^R145G/+ cardiomyocytes. J, Time of 90% decay (T90_off) is assessed to be longer in TnnI3^R145G/+ cardiomyocytes by CalTrack analysis. K, Calcium decay constant (Tau) is significantly longer in TnnI3^R145G/+ cardiomyocytes. Statistical analysis is performed by a 2-tailed Student t test (Baseline, T_on, T90_on, and T90_off) or by nonparametric Mann-Whitney test (CaAmplitude, T10_on, T50_on, T10_off, T50_off, tau) with a significance cutoff of P<0.05. No statistical comparisons are made between IonOptix and CalTrack. Ns indicates nonsignificance.