Figure 3.

Pattern of antigenic responses after expansion of SARS-CoV-2 T cells from COVID-19-RDs compared with baseline

(A) Bar graph showing the percentages of CD4+ and CD8+ subsets of SARS-CoV-2 T cells at baseline.

(B) Graphical analysis showing the percentages of IFN-γ (+) SARS-CoV-2 T cells from RDs at baseline in the CD4+ compartment (left panel) or CD8+ compartment (right panel) when stimulated with the peptide libraries derived from the different structural proteins M (blue), N (red), and S (green).

(C and D) Pie charts illustrating the percent distribution of IFN-γ (+) CD4 and CD8 T cells reactive to M (blue), N (red), S1 (dark green), and S2 (light green) peptide libraries at baseline (C) or following expansion with IL-2/4/7 (top panel) or IL-2/7/15 (bottom panel) cytokine cocktails (D).

The bars represent median values with interquartile ranges. Statistical analysis by paired t test (A and B).