Table 1.
Cytokine production of SARS-CoV-2 T cells from RDs expanded with different cytokine cocktails against the M, N, and S structural proteins
| CD3% |
CD4% |
CD8% |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| M | N | S | M | N | S | M | N | S | ||
| IL-2/4/7 | IFN-γ median | 4.27 | 4.98 | 10.60 | 4.24 | 5.88 | 10.65 | 2.42 | 4.81 | 0.82 |
| IFN-γ min | 0.11 | 0.12 | 0.21 | 0.14 | 0.14 | 0.22 | 0.10 | 0.05 | 0.25 | |
| IFN-γ max | 33.60 | 17.10 | 14.80 | 33.40 | 18.90 | 20.10 | 42.00 | 19.40 | 3.99 | |
| IL-2 median | 0.68 | 1.01 | 0.96 | 0.41 | 0.70 | 0.90 | 0.42 | 0.42 | 0.27 | |
| IL-2 min | 0.14 | 0.23 | 0.20 | 0.14 | 0.18 | 0.16 | 0.00 | 0.16 | 0.18 | |
| IL-2 max | 1.41 | 1.85 | 2.31 | 1.44 | 2.09 | 1.82 | 1.20 | 0.71 | 0.79 | |
| TNF-α median | 2.80 | 2.35 | 3.99 | 2.64 | 2.09 | 4.59 | 1.45 | 0.97 | 0.61 | |
| TNF-α min | 0.19 | 0.22 | 0.25 | 0.05 | 0.04 | 0.07 | 0.10 | 0.00 | 0.09 | |
| TNF-α max | 12.90 | 7.50 | 7.86 | 12.90 | 8.41 | 7.90 | 5.65 | 3.70 | 2.42 | |
| IL-2/7/15 | IFN-γ median | 6.05 | 3.60 | 4.47 | 6.00 | 3.61 | 4.55 | 0.33 | 3.13 | 1.15 |
| IFN-γ min | 0.15 | 0.17 | 0.48 | 0.13 | 0.21 | 0.42 | 0.09 | 0.04 | 0.12 | |
| IFN-γ max | 20.80 | 15.50 | 26.00 | 34.20 | 16.10 | 29.40 | 47.30 | 20.50 | 2.65 | |
| IL-2 median | 1.03 | 0.53 | 0.79 | 0.68 | 0.58 | 0.80 | 0.37 | 0.49 | 0.41 | |
| IL-2 min | 0.15 | 0.32 | 0.31 | 0.20 | 0.28 | 0.22 | 0.20 | 0.23 | 0.27 | |
| IL-2 max | 2.55 | 1.56 | 6.59 | 1.64 | 0.91 | 6.52 | 2.24 | 1.16 | 1.75 | |
| TNF-α median | 4.18 | 1.70 | 2.37 | 3.90 | 1.46 | 2.74 | 0.69 | 1.29 | 0.76 | |
| TNF-α min | 0.24 | 0.38 | 0.78 | 0.10 | 0.48 | 0.33 | 0.00 | 0.10 | 0.24 | |
| TNF-α max | 7.06 | 4.20 | 10.10 | 12.60 | 4.50 | 11.20 | 18.50 | 7.27 | 3.61 | |
| IL-2/4/21 | IFN-γ median | 0.30 | 0.20 | 0.36 | 0.51 | 0.29 | 0.16 | 0.00 | 0.00 | 0.00 |
| IFN-γ min | 0.03 | 0.14 | 0.08 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
| IFN-γ max | 2.46 | 0.64 | 2.53 | 4.99 | 1.08 | 3.63 | 3.37 | 0.22 | 0.97 | |
| IL-2 median | 0.16 | 0.21 | 0.16 | 0.13 | 0.20 | 0.16 | 0.00 | 0.00 | 0.00 | |
| IL-2 min | 0.10 | 0.09 | 0.09 | 0.00 | 0.09 | 0.06 | 0.00 | 0.00 | 0.00 | |
| IL-2 max | 0.40 | 0.84 | 1.02 | 0.52 | 0.33 | 0.81 | 0.24 | 0.48 | 2.17 | |
| TNF-α median | 0.40 | 0.15 | 0.14 | 0.34 | 0.20 | 0.14 | 0.00 | 0.00 | 0.00 | |
| TNF-α min | 0.10 | 0.09 | 0.00 | 0.06 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
| TNF-α max | 0.93 | 0.47 | 1.66 | 1.95 | 0.50 | 2.36 | 3.70 | 7.69 | 0.00 | |
| IL-2/7/21 | IFN-γ median | 0.74 | 0.25 | 0.29 | 1.27 | 0.16 | 0.18 | 0.00 | 0.33 | 0.41 |
| IFN-γ min | 0.14 | 0.06 | 0.11 | 0.07 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
| IFN-γ max | 0.97 | 0.38 | 0.50 | 2.93 | 0.81 | 1.36 | 1.52 | 5.82 | 1.11 | |
| IL-2 median | 0.09 | 0.07 | 0.11 | 0.20 | 0.22 | 0.14 | 0.10 | 0.13 | 0.34 | |
| IL-2 min | 0.03 | 0.05 | 0.07 | 0.10 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
| IL-2 max | 0.73 | 0.66 | 0.31 | 1.27 | 1.10 | 0.57 | 1.75 | 0.60 | 0.83 | |
| TNF-α median | 0.19 | 0.19 | 0.20 | 0.45 | 0.33 | 0.59 | 0.39 | 0.53 | 0.10 | |
| TNF-α min | 0.13 | 0.07 | 0.14 | 0.17 | 0.19 | 0.18 | 0.00 | 0.00 | 0.00 | |
| TNF-α max | 1.70 | 0.31 | 0.26 | 2.55 | 1.01 | 2.58 | 0.94 | 0.72 | 0.93 | |
Shown are percent IFN-γ, IL-2, and TNF-α production (median, minimum [min], and maximum [max] values) from the CD3+, CD4+, and CD8+ compartments of SARS-CoV-2 T cells stimulated with the peptide libraries derived from the M, N, and S structural proteins after expansion under the different culture conditions with the four different cytokine cocktails IL-2/4/7, IL-2/7/15, IL-2/4/21, and IL-2/7/21.