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. 2021 Jul 6;17(7):66. doi: 10.1007/s11306-021-01815-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Reorganized and B not reorganized hierarchical clustering and heatmaps of the 13 metabolites that were different in their peak intensity before and after weight reduction. Ordered columns represent the fecal metabolite profile for each dog before (T0) and after a period of weight reduction (T1) using a therapeutic diet and sorted by group. Colored bars on the x-axis represent group of dogs (red, obese dogs before weight loss; blue, obese dogs after weight loss). Rows represent the 13 metabolites that differed, highlighting two clusters of metabolites: the first comprised metabolites that decreased after weight reduction (i.e., purine, 1-methylxanthine, coumestrol, trigonelline, L-(-)-methionine, and cetrimonium); the second comprised metabolites that increased after weight reduction (i.e., imidacloprid, 1,5-isoquinolinediol, 2-hydroxiquinoline, 5-fluoro-3,5-AB-PFUPPYCA, matairesinol, 4-hydroxybenzaldehido, and (-)-epicatechin)