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. 2021 Jul 6;12:4160. doi: 10.1038/s41467-021-24299-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Diazotroph abundance. The percentage of diazotrophs in the bacterioplankton community was estimated by the ratio of metagenomic read abundance between the marker genes nifH and recA. b Taxonomic distribution of the nifH reads. c Taxonomic distribution at deeper resolution. HBD01 to HBD09 (heterotrophic bacterial diazotrophs) corresponds to the metagenome-assembled genomes from <3-µm size fractions¹⁶. Boxplots depict the 25–75% quantile range of the dataset without zeros (the corresponding biologically independent seawater samples are indicated in the plot), with the center line depicting the median (50% quantile); whiskers encompass data points within 1.5× the interquartile range. Source data are provided as a Source data file.