Figure 3.

Dialysis recoveries of AChE inhibitions by 3, 4, and 5 (A), and of MAO-B inhibitions by 3 and 5 (B). The concentrations of 3, 4, 5, and tacrine used were 25, 20, 80, and 0.60 µM, respectively, and the concentrations of 3, 5, lazabemide, and pargyline used were 14, 20, 0.28, and 0.06 µM, respectively. Tacrine was used as a reference reversible AChE inhibitor, and lazabemide and pargyline were used as reference reversible and irreversible MAO-B inhibitors, respectively. After preincubating the compounds with enzymes for 15 min, the mixtures were dialyzed for 6 h with two buffer changes. Results are the averages of duplicate or triplicate experiments.