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. 2021 Jul 6;12:4137. doi: 10.1038/s41467-021-24412-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Principal component analysis of monocytes derived from CB (CB-mono) and PBSC (PBSC-mono). Red, CB-mono; blue, PBSC-mono. Each point represents a biologically independent sample. b Heat map representation of differentially-expressed genes (fold change >2, adjusted P < 0.05) between CB-mono and PBSC-mono. Each column represents an individual biological replicate. c Heat map representation of the expression of key proinflammatory and regulatory genes in CB-mono and PBSC-mono. Each column represents an individual biological replicate. d Comparison of microarray expression values in CB-mono and PBSC-mono. e GSEA plots showing that the activation of immune-response genes was upregulated in CB-mono. f GSEA plots showing that positive regulation of peptide-secretion genes was upregulated in CB-mono. g GM-CSF, IL-6, and TNF-α expression in CB-mono or PBSC-mono (p = 0.0476, 0.0022, and 0.0022, respectively), as assessed by RT-PCR. Data are representative of six experiments. Mann–Whitney test (two-sided). h Representative histograms of intracellular IL-6 in monocytes, T cells, NK cells, and B cells from peripheral blood stem cells or cord blood; n = 16 for peripheral blood stem cells; n = 17 for cord blood. i Representative histograms of intracellular TNF-α in monocytes, T cells, NK cells, and B cells from peripheral blood stem cells or cord blood; n = 12 for peripheral blood stem cells; n = 15 for cord blood. j Flow cytometry gating strategy utilized to identify GM-CSF⁺ cells; PBSC, n = 6; CB, n = 12. The numbers in (h) and (i) show the mean fluorescence intensity (MFI) of IL-6 and TNF-α expression, respectively. Data in (g) are presented as means ± standard error of the mean (SEM). *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.