Fig. 3. Strategy for constructing HEK 293T cell lines with a landing pad and screening the 5′ UTR library using recombinase-based gene integration.

a Recombinase-based library screening workflow. b Construction of the 5′ UTR library and schematic illustration of recombinase-based gene integration. c We observed high reproducibility for barcode representations between two HEK-LP cell lines independently transfected with the library and a recombinase-expression plasmid; cells were sorted into three bins based on GFP expression (top 0–2.5%, top 2.5–5%, and top 5–10%). log2 values of normalized barcode counts are shown. R is the Pearson correlation coefficient.