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. 2021 Jul 6;11:13912. doi: 10.1038/s41598-021-93113-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PD-L1 expression and CD8α⁺ infiltration in FM3A tumor model. (A) PD-L1 immunohistochemical staining in tumor tissue at baseline in the Colon 38 model (positive control) and FM3A model. (B) CD8α immunohistochemical staining in tumor tissue at baseline. (C) PD-L1 expression on tumor-infiltrating immune cells and tumor cells in FM3A tumors. (D) Distribution of cells in FM3A tumors. Cells were determined by flow cytometric analysis.