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. 2021 Jul 6;12:4147. doi: 10.1038/s41467-021-24380-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Luciferase activity of the ETS responsive reporter in LNCaP cells expressing WT, K362A, K362A/E412A ERG, or empty vector (EV). Bottom, protein expression verified by immunoblotting. b Luciferase activity of the ETS responsive reporter in RWPE1 cells expressing WT, K362A, E412A, K362A/E412A ERG, or empty vector (EV). Bottom, protein expression verified by immunoblotting. c Total modulated genes in LNCaP cells expressing WT or K362A ERG. d Heat map showing the intensity of the transcripts significantly modulated in LNCaP cells expressing WT, K362A ERG, or empty vector (EV). Scale bar shows LogRatio range. Red, upregulation; green, downregulation (n = 2/group). e Scatter plot of the LogRatios of genes deregulated in LNCaP-ERG vs control (logFC ERG WILD) and LNCaP-ERG-K362A vs control (logFC ERG MUT). Regression line was calculated by means of the lm function (regression line coefficient = 0.44). f Venn diagram showing the convergence of genes modulated by WT-ERG and ERG-K362A. g Convergence between genes modulated by WT-ERG or ERG-K362A and ERG genomic occupancy in VCaP cells. h Soft agar, clonogenic, and sphere forming assays in LNCaP cells expressing WT, K362A ERG or an empty vector (EV). i Growth of xenografts of LNCaP cells expressing WT-ERG, ERG-K362A, or an empty vector (EV) injected in NSG mice (n = 3/group). Right, histological and Ki67 immunostaining scores in tumor xenografts. Scale bars represent 200 µm. j Growth of xenografts of RWPE1 cells expressing WT, ERG-K362A, K362A/E412A (DM) ERG, or an empty vector (EV) injected in NSG mice (n = 4, biological independent samples). Right, histological and Ki67 immunostaining scores in tumor xenografts. Scale bars represent 200 µm. All error bars, mean ± s.d. P-values were determined by one-way ANOVA test. Source data are provided as a Source Data File.