Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 6;12:4147. doi: 10.1038/s41467-021-24380-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a ChIP analysis of ERG and EZH2 occupancy on the IL-6 promoter in VCaP cells. Data are presented as fold enrichment relative to input. b ChIP–reChIP analysis of ERG and EZH2 to evaluate co-occupancy at the IL-6 promoter in VCaP cells. Data are presented as fold enrichment relative to input. c qRT-PCR analysis of IL-6 mRNA and immunoblotting analysis of the indicated proteins (right) upon ERG and EZH2 knockdown in VCaP cells. d qRT-PCR analysis of IL-6 mRNA (left) and IL-6 promoter activity by luciferase reporter assay (middle) in LNCaP cells with ERG overexpression and EZH2 knockdown. Expression of the indicated proteins was verified by immunoblotting (right panel). e ChIP analysis of ERG and EZH2 occupancy in the IL-6 promoter at the ETS binding site (EBS) in LNCaP and LNCaP-ERG stable cell lines. f IL-6 mRNA determined by qRT-PCR (left) and IL-6 promoter activity by luciferase reporter assay (middle) in LNCaP transfected with the indicated plasmids. Expression of the indicated proteins was verified by immunoblotting (right). g ChIP–reChIP analysis of EZH2, ERG, and SUZ12 to evaluate co-occupancy at the IL-6 and Nkx3.1 promoters in VCaP cells. Data are presented as fold enrichment relative to IgG of the Re-ChIP. All error bars, mean ± s.d. (n = 3, technical replicates). P-values were determined by one-way ANOVA test. Source data are provided as a Source Data File.