TABLE 1.

Primers used in the study.

Target gene (primer)	Primer type	Primer sequences (5′–3′)	Reference
16S–23S rRNA Internal Transcribed Spacer (ITS Ab1)	ITS Ab1 F3 a	CAC​AAC​AAG​TTG​TTC​TTC​ATA​GAT	This study
	ITS Ab1 B3 a	CGAACTCCTGACCTCCTG
	ITS Ab1 FIP	AGA​CTT​GAA​CTT​GTG​ACC​CCA​CTG​AGG​GTC​TGT​AGC​TCA​G
	ITS Ab1 BIP	ACCATGACTTTGACTGGTTRAAGTTCGCTCTACCAACTAAGCTAAG
	ITS Ab1 LF	TCA​AGC​GTG​TGC​TCT​AAC​C
	ITS Ab1 LB	GATACATGAYTGATGATGTAAGCTG
16S–23S rRNA Internal Transcribed Spacer (ITS Ab2)	ITS Ab2 F3 a	AGA​TGT​ATC​TGA​GGG​TCT​GT	This study
	ITS Ab2 B3 a	TTCTGGTGGAGACTAGGA
	ITS Ab2 FIP	ATG​GTG​GGT​CTG​ACA​AGA​CTT​GTC​AGT​TGG​TTA​GAG​CAC​AC
	ITS Ab2 BIP	ACATGAYTGATGATGTAAGCTGGGGGTCGAACTCCTGACCTC
	ITS Ab2 LF	GAT​AAG​CGT​GGG​GTC​ACA​AG
	ITS Ab2 LB	CAG​GCG​CTC​TAC​CAA​CTA​AGC
bla OXA-23 (OXA-23)	OXA-23 F3 a	GAA​GCC​ATG​AAG​CTT​TCT​G	Yamamoto N et al., 2015
	OXA-23 B3 a	GTA​TGT​GCT​AAT​TGG​GAA​ACA
	OXA-23 FIP	ACC​GAA​ACC​AAT​ACG​TTT​TAC​TTC​TCA​GTC​CCA​GTC​TAT​CAG​GA
	OXA-23 BIP	CTG​AAA​TTG​GAC​AGC​AGG​TTG​ACT​CTA​CCT​CTT​GAA​TAG​GCG
	OXA-23 LF	TTT​TGC​ATG​AGA​TCA​AGA​CCG​A
	OXA-23 LB	CTG​GTT​GGT​AGG​ACC​ATT​AAA​GGT​T

F3, forward outer primer; B3, backward outer primer; FIP, forward inner primer; BIP, backward inner primer; LF, loop forward primer; LB, loop backward primer. MP primers are used for amplification of ITS 16S–23S rRNA for identification of clinically significant members of the Acinetobacter calcoaceticus–baumannii (ACB) complex and bla_OXA-23 gene for detection of carbapenem resistance.

^aForward and backward outer primers (F3 and B3) of each set of LAMP primers were used for PCR.