TABLE 2.
Details of bacterial strains used in the study.
| Details of bacterial strains | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Non-Acinetobacter species | No. | Type | ||||||||
| Staphylococcus aureus ATCC 25923 | 1 | Reference | ||||||||
| Enterococcus faecalis ATCC 29212 | 1 | |||||||||
| Escherichia coli ATCC 25922 | 1 | |||||||||
| Klebsiella pneumoniae ATCC BAA 1705 | 1 | |||||||||
| Enterobacter cloacae ATCC 700323 | 1 | |||||||||
| Candida krusei ATCC 6258 | 1 | |||||||||
| Pseudomonas aeruginosa ATCC 27858 | 1 | |||||||||
| Serratia marcescens | 1 | Clinical | ||||||||
| Providencia stuartii | 1 | |||||||||
| Stenotrophomonas maltophilia | 1 | |||||||||
| Acinetobacter species | ||||||||||
| ACB complex | Acinetobacter baumannii ATCC 19606 | 1 | Reference | |||||||
| Acinetobacter pittii a | 6 | Clinical | ||||||||
| Acinetobacter nosocomialis b | 2 | |||||||||
| Carbapenem-resistant A. baumannii (CRAB/05/RICU/VMMC-SJH); a pre-characterized clinical isolate positive for bla OXA-23 | 1 | |||||||||
| Thirty diverse genotypes of Acinetobacter baumannii | ||||||||||
| Pulsotypes | Genotypic characterization | Phenotypic characterization | ||||||||
| bla OXA-51 | bla OXA-23 | bla NDM-1 | Biofilm production | AST results | ||||||
| MER | IMP | NET | ||||||||
| I | + | + | + | ++ | R | R | R | 1 | ||
| II | + | + | + | ++ | R | R | I | 1 | ||
| III | + | + | + | + | R | R | R | 1 | ||
| IV | + | + | − | +++ | R | R | R | 1 | ||
| V | + | + | − | +++ | I | I | S | 1 | ||
| VI | + | + | − | ++ | R | R | R | 5 | ||
| VII | + | + | − | ++ | R | I | R | 1 | ||
| VIII | + | + | − | + | R | R | S | 3 | ||
| IX | + | + | − | + | R | R | R | 11 | ||
| X | + | − | + | + | R | R | R | 1 | ||
| XI | + | − | + | − | R | R | S | 1 | ||
| XII | + | − | − | + | S | S | S | 2 | ||
| XIII | + | − | − | + | R | R | R | 1 | ||
| Non-ACB complex | Acinetobacter lwoffii | 6 | Clinical | |||||||
| Acinetobacter variabilis | 3 | |||||||||
| Acinetobacter ursingii | 2 | |||||||||
| Acinetobacter radioresistens | 2 | |||||||||
| Acinetobacter junii | 2 | |||||||||
| Acinetobacter schindleri | 2 | |||||||||
| Acinetobacter indicus | 2 | |||||||||
| Acinetobacter haemolyticus | 1 | |||||||||
| Acinetobacter bereziniae | 1 | |||||||||
a
Two strains with distinct MLST patterns and four from patients from diverse geographical areas in India.
b
Clinical strains isolated in 2018 and 2021; ATCC, American Type Culture Collection; ACB complex, Acinetobacter calcoaceticus–baumannii complex; AST, antimicrobial sensitivity testing; MER, meropenem; IMP, imipenem; NET, netilmicin; R, resistant; S, sensitive; I, intermediate. In the genotypic characterization section, + and – are indicative of the presence and absence of gene, respectively. In the biofilm production section: −, +, ++, and +++ are indicative of none, weak, moderate, and strong biofilm producer, respectively.