TABLE 3.
Limit of detection of LAMP and PCR assays from different DNA concentrations and colony counts using ITS (Ab1 and Ab2) and OXA-23 primers for detection of clinically significant members of the ACB complex and carbapenem resistance, respectively.
| Primers | Limit of detection of LAMP and PCR assays | |||
|---|---|---|---|---|
| DNA concentration (/µl) | Colony count (cfu/ml) | |||
| LOD of LAMP with detection time | LOD of PCR | LOD of LAMP with detection time | LOD of PCR | |
| ITS Ab1 | 100 ng (20 min) | 1 ng | 108 (20 min) | 108 |
| 107 (25 min) | ||||
| 10 ng (20 min) | 106 (25 min) | |||
| 1 ng (25 min) | 105 (30 min) | |||
| 100 pg (30 min) | 104 (30 min) | |||
| ≤10 pg (ND till 45 min) | ≤103 (ND till 45 min) | |||
| ITS Ab2 | 100 ng (45 min) | 1 ng | 108 (50 min) | 108 |
| 10 ng (50 min) | ||||
| 1 ng (50 min) | ≤107 (ND till 60 min) | |||
| 100 pg (50 min) | ||||
| ≤10 pg (ND till 60 min) | ||||
| OXA-23 | 100 ng (10 min) | 100 pg | 108 (10 min) | 108 |
| 10 ng (10 min) | 107 (10 min) | |||
| 1 ng (10 min) | 106 (15 min) | |||
| 100 pg (15 min) | 105 (15 min) | |||
| 10 pg (15 min) | 104 (15 min) | |||
| 1 pg (15 min) | 103 (15 min) | |||
| ≤100 fg (ND till 30 min) | ≤102 (ND till 30 min) | |||
ACB complex, Acinetobacter calcoaceticus–baumannii complex; LOD, limit of detection; min, minutes; ND, not detected; cfu/ml, colony-forming unit/ml.