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. 2021 Jun 23;11:647175. doi: 10.3389/fonc.2021.647175

Figure 2.

Figure 2

Overexpression of IRAK2 restored radiosensitivity via enhancing radiation-induced apoptosis in OML1-R cells. (A) Colony formation assay showed that IRAK2-overexpressed OML1-R cells restored their radiosensitivity when compared with that of control OML1-R cells (P = 0.0100). (B) Apoptosis-specific flow cytometry represented that overexpression of IRAK2 significantly enhanced apoptosis in OML1-R cells before (52.28% vs. 0.62%) and after (63.50% vs. 0.81%) 4-Gy IR. The histogram on the right represent Annexin V-positive staining enrichment. (C) In OML1-R cells, protein levels of apoptosis-related factors, i.e., cleaved caspase-8, cleaved caspase-3, CHOP, and p65-NF-κB, were elevated by the overexpression of IRAK2, especially after 4-Gy IR (Western blotting, 72 hours after IR). (D) Protein levels of IRAK2, cleaved caspase-8 and cleaved caspase-3 were analyzed for OML1-R cells treated with an IRAK2 overexpression followed by the pretreatment with caspase-8 inhibitors (50 mM Z-IETD-FMK) for 1 hour. Densitometry-derived values (bottom) were normalized with the control set as 1. β-actin served as the loading control for normalization.