FIGURE 4.

CfGcn5 is involved in germination and appressoria formation. (A) The conidia of WT, ΔCfgcn5, and ΔCfgcn5/CfGCN5 were inoculated on artificial hydrophobic surfaces and onion epidermis, respectively. Germination and appressoria formation were observed after inoculation on hydrophobic surfaces at different time points (2, 8, and 24 h), and on onions epidermis (24 h). Asterisks indicate germ tubes, black arrows indicates appressoria, and white arrows indicates conidia. Bar = 10 μm. (B) Statistical analysis of germination and appressoria formation rates. Error bars represent standard deviations (SD) of three replicates and asterisks indicate significant differences (p < 0.01). The experiments were repeated three times with three replicates each time, and more than 100 conidia were counted per replicate.