FIGURE 6.

CfGcn5 plays roles in cell wall integrity. (A) The WT, ΔCfgcn5, and ΔCfgcn5/CfGCN5 were incubated on PDA plates with various cell wall stresses of CFW, CR, and SDS at 28°C for 4 days. (B) Statistical analysis of inhibition rates of the strains to cell wall stresses and asterisks indicate significant differences (p < 0.01). (C) The mycelia of the strains were stained with 10 μg/mL CFW for 5 min without light before being photographed, arrows indicates the stained hyphal tips. The experiment was repeated three times with triplicates, which showed the same results. DIC, differential interference contrast image. (D) Light microscopic observation of protoplast release after treatment with cell wall-degrading enzymes for 2 h at 30°C, arrows indicates the non-degraded hypha. Bar = 10 μm.