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. 2021 Jul 6;12:4142. doi: 10.1038/s41467-021-24266-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a A phylogenetic tree analysis of the potatoes S-RNases. StS-RNase1–14 are 14 types of S-RNases reported in this study (indicated in red), while the other 35 S-RNases were obtained from GenBank. b Yeast two-hybrid (Y2H) analysis of the interactions between 14 potential S-RNases and the PP2 domain of Sli (BD-PP2). The conserved domains of Sli are indicated by rectangles. The interactions between potential S-RNases and the full length of Sli are shown in Supplementary Fig. 6. The F-box protein interaction domain of a SLF gene (RHC01H2G1617) was used as a negative control [BD-StSLF(t)]. WLHA, synthetic dropout media lacking tryptophan, leucine, histidine, and adenine. BD binding domain, AD activation domain. c The firefly luciferase (LUC) complementation imaging assays of the interactions between the S-RNases and the PP2 domain of Sli. LUC signal was captured in the leaf area co-expressing the PP2 domain of Sli and each of the S-RNases (indicated in red), except for S3, S9, S10, and S13. Sn denote each type of the StS-RNase1–14, nLUC the N terminal of LUC protein, cLUC the C terminal of LUC protein. The experiments were repeated three times with similar results.