Fig 1.

Study characteristics. (A) Average running distance by mice assigned to physical activity. (B) Experimental process and animal euthanization time point and groups. (C) Specially designed individual mouse cage with an attached monitoring device, where mice can voluntary access running wheels. (D) Average running time for mice assigned to physical activity. (E) Average body weights of each group of mice from the experimental start point at 4 weeks of age to the end at 20 weeks of age. (F) Average fat and lean mass of each group of mice at second euthanization time point at 16 weeks of age. (G) Average fat and lean mass of each group of mice at third euthanization time point at 20 weeks of age. ***p < 0.001 by two‐way ANOVA with a HFD and early‐life PA as the main factors and their interactions. AVG, average; Con, control; HFD, high‐fat diet; PA, physical activity; sac'd, sacrificing day.