Fig 4.

Short‐term early‐life–increased physical activity (PA) does not prevent later life high‐fat diet (HFD)–induced bone loss. (A) Representative μCT images of the proximal tibia from one sample from each group of mice. Upper panel shows sagittal view and lower panel shows transverse view; white lines and dots indicate trabecular or cortical bone tissues. (B–G) μCT measures of six parameters from trabecular tibias from no PA/control, PA/control, no PA/HF, and PA/HF of 16‐week‐old mouse groups. Data are expressed as mean ± SD (n = 6 per group), analyzed by two‐way ANOVA with a HFD and early‐life PA as the main factors, and their interactions were tested. Additionally, *p < 0.05, **p < 0.01, ***p < 0.001 by Tukey's multiple comparison. (H) Representative images of transverse views of quantitative pQCT analysis of one slice of the proximal tibia from one sample from each group of mice. Color changes from black to white indicate bone density from low to high. BS/TV, bone surface density; BV/TV, bone volume/total tissue volume; HF, high fat; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Th, trabecular thickness; SMI, structure model index; SSI, stress–strain index.