Table 2.
Primers used for quantitative real time PCR analysis of chicken mRNAs.
| Gene | Primer sequence (5′-3′) | GC (%) | Tm (°C) | PCR Product (bp) | Accession No. |
|---|---|---|---|---|---|
| ACTC1 | TGTGACGACGAGGAGACCAC | 60 | 61.51 | 110 | NM_001079481.1 |
| CCGACGATGGATGGGAACAC | 60 | 60.81 | |||
| MHCα | CAGGTGCAATCCCAGAGGAC | 60 | 60.39 | 246 | NM_204766.2 |
| CCCTGCGCTCCACTATCTTC | 60 | 60.25 | |||
| MHCβ | GAGGAGGAGTGCATGTTCCC | 60 | 60.11 | 148 | NM_001001302.1 |
| CCGCGTAGTGGATGAGTGAG | 60 | 60.25 | |||
| RYR2 | CCCTGAAGCTAGGGATTGCC | 60 | 60.18 | 199 | XM_025149046.1 |
| CCCTCTTCAGTCACCATGCC | 60 | 60.39 | |||
| SERCA2 | GTCTTCCCTCCGTGGAAACC | 60 | 60.32 | 170 | M66385.1 |
| GGGAGCGTATGTCGAACCAG | 60 | 60.53 | |||
| THRα | AGACGTACCTGCTGGCGTTC | 60 | 62.49 | 150 | NM_205313.2 |
| GGCACTCCACCTTCATGTGC | 60 | 61.59 | |||
| THRβ | GGCCTTGTTTGCGTCGAGAG | 60 | 61.90 | 187 | X17504.1 |
| GGCACTCCACCTTCATGTGC | 60 | 61.59 | |||
| Troponin | TGGATGAGGAGCGCTACGAC | 60 | 61.73 | 261 | NM_213570.1 |
| CCACGTTCTTCCGCCAATCG | 60 | 61.97 | |||
| YWHAZ | AGGAGCCGAGCTGTCCAATG | 60 | 62.25 | 83 | NM-001031343.1 |
| TCCAAGATGACCTACGGGCTC | 57 | 61.30 |