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. 2021 May 4;100(8):101240. doi: 10.1016/j.psj.2021.101240

Table 2.

Broiler mean BW, relative weight of the whole small intestine (SI), liver, spleen, bursa, duodenum, jejunum, and ileum at 0, 7, 14, and 40 d of age (doa) in diluent in ovo-injected (50 μL) control eggs, and eggs in ovo-injected with diluent containing 2.4 μg of vitamin D3 (D3) or 25-hydroxycholecalciferol (25OHD3) alone, or a combination of 2.4 μg of D3 and 2.4 μg of 25OHD3 (D3 + 25OHD3), and fed a commercial diet or a diet in which calcium and available phosphorus were restricted by 20%.

Treatment BW (g) SI1 (%) Liver (%) Spleen (%) Bursa (%)
0 doa
In ovo injection
 Diluent2 41.3 2.88 1.02 0.017 0.087
 D33 41.0 3.38 0.99 0.015 0.143
 25OHD34 41.8 2.82 0.95 0.023 0.041
 D3+25OHD35 40.6 2.99 0.98 0.016 0.093
Pooled SEM 0.78 0.191 0.036 0.0029 0.0528
P value 0.759 0.177 0.588 0.158 0.599
Treatment BW (g) Duodenum (%) Jejunum (%) Ileum (%) Liver (%) Spleen (%) Bursa (%)
7 doa
In ovo injection
 Diluent 158.8 1.63 2.83 2.46 3.12 0.076 0.36
 D3 156.8 1.77 2.54 2.62 3.18 0.077 0.17
 25OHD3 147.8 2.16 2.81 2.62 3.15 0.084 0.26
 D3+25OHD3 157.4 1.52 2.75 2.29 3.08 0.087 0.17
Diet
 Commercial 153.5 1.88 2.74 2.62 3.08 0.077 0.16
 ReCaP6 156.9 1.67 2.73 2.45 3.18 0.085 0.32
Pooled SEM 4.55 0.123 0.300 0.193 0.155 0.007 0.112
P value
In ovo 0.413 0.072 0.845 0.539 0.952 0.634 0.566
Diet 0.506 0.230 0.966 0.510 0.437 0.226 0.142
In ovo x Diet 0.177 0.132 0.742 0.881 0.451 0.887 0.530
Treatment BW (g) Duodenum (%) Jejunum (%) Ileum (%) Liver (%) Spleen (%) Bursa (%)
14 doa
In ovo injection
 Diluent 452 1.85 1.87 1.58 2.68 0.106 0.493
 D3 449 1.15 1.76 1.53 2.93 0.169 0.178
 25OHD3 465 1.10 1.62 1.42 2.53 0.139 0.210
 D3+25OHD3 445 1.27 1.61 1.63 2.75 0.069 0.153
Diet
 Commercial 454 1.46 1.63 1.53 2.73 0.149 0.188
 ReCaP 450 1.23 1.80 1.55 2.72 0.092 0.161
 Pooled SEM 9.8 0.255 0.084 0.110 0.070 0.0477 0.021
P value
In ovo 0.563 0.439 0.241 0.514 0.055 0.634 0.493
Diet 0.731 0.524 0.087 0.891 0.875 0.318 0.370
In ovo x Diet 0.285 0.280 0.399 0.463 0.061 0.327 0.481
Treatment BW (g) Duodenum (%) Jejunum (%) Ileum (%) Liver (%) Spleen (%) Bursa (%)
40 doa
In ovo injection
 Diluent 2,408 0.497 1.025 0.738 2.26 0.100 0.118
 D3 2,261 0.564 1.038 0.818 2.14 0.091 0.145
 25OHD3 2,402 0.532 0.963 0.755 2.14 0.090 0.138
 D3+25OHD3 2,254 0.510 1.021 0.657 2.50 0.112 0.119
Diet
 Commercial 2,573a 0.476b 0.969 0.718 2.30 0.096 0.125
 ReCaP 2,089b 0.575a 1.055 0.766 2.22 0.101 0.136
Pooled SEM 69.3 0.0250 0.044 0.0549 0.088 0.0055 0.0120
P value
In ovo 0.228 0.575 0.838 0.350 0.260 0.290 0.342
Diet 0.001 0.013 0.204 0.415 0.566 0.537 0.422
In ovo x Diet 0.114 0.819 0.635 0.389 0.518 0.475 0.379

Treatment means within the same variable column within type of treatment with no common superscript differ significantly (P < 0.05).

1

The weight of and gastrointestinal tract from the bottom of proventriculus to the end of the ceca.

2

Eggs injected with 50 μL commercial diluent at d 18 of incubation.

3

Eggs injected with 50 μL commercial diluent containing vitamin D3 at 2.4 μg at d 18 of incubation.

4

Eggs injected with 50 μL commercial diluent containing 25OHD3 at 2.4 μg at d 18 of incubation.

5

Eggs injected with 50 μL commercial diluent containing D3 at 2.4 and 25OHD3 at 2.4 μg at d 18 of incubation.

6

A diet restricted Ca and available P by 20% throughout the rearing period.