. 2021 May 4;100(8):101240. doi: 10.1016/j.psj.2021.101240

Table 3.

Broiler mean plasma IgG at 7 and 14 d of age (doa), IgM at 14 doa, and alpha-1-acid glycoprotein (AGP) at 40 doa in diluent in ovo-injected (50 μL) control eggs, and eggs in ovo-injected with diluent containing 2.4 μg of vitamin D₃ (D₃) or 25-hydroxycholecalciferol (25OHD₃) alone, or a combination of 2.4 μg of D₃ and 2.4 μg of 25OHD₃ (D₃ + 25OHD₃), and fed a commercial diet or a diet in which calcium and available phosphorus were restricted by 20%.

Treatment	IgG-7doa¹	IgG-14doa²	IgM-14doa³	AGP-40doa⁴
μM
In ovo injection
Diluent⁵	2.39	6.52^bc	2.37^c	2.92
D₃⁶	2.26	6.43^c	2.42^bc	2.93
25OHD₃⁷	2.34	6.63^a	2.48^ab	2.72
D₃+25OHD₃⁸	2.34	6.58^ab	2.57^a	2.86
Diet⁹
Commercial	2.32	6.58	2.42	2.86
ReCaP	2.35	6.50	2.50	2.82
Pooled SEM	0.049	0.053	0.045	0.009
P value
In ovo	0.302	0.013	0.005	0.079
Diet	0.570	0.090	0.080	0.580
In ovo x Diet	0.302	0.984	0.974	0.129

^a,bTreatment means within the same variable column within type of treatment with no common superscript differ significantly (P < 0.05).

Plasma IgG concentrations at 7 doa.

Plasma IgG concentrations at 14 doa.

Plasma IgM concentrations at 14 doa.

⁴

Plasma AGP concentrations at 40 doa.

⁵

Eggs injected with 50 μL commercial diluent at d 18 of incubation.

⁶

Eggs injected with 50 μL commercial diluent containing vitamin D₃ at 2.4 μg at d 18 of incubation.

⁷

Eggs injected with 50 μL commercial diluent containing 25OHD₃ at 2.4 μg/ at d 18 of incubation.

⁸

Eggs injected with 50 μL commercial diluent containing D₃ at 2.4 and 25OHD₃ at 2.4 μg at d 18 of incubation.

⁹

A diet restricted Ca and available P by 20% through the rearing period.