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Schema of the synthesis of PAR28-phospholipids and preparation of PAR28-displaying liposomes. PAR28-PEG-DSPE (A) and PAR28-DSPE (B) were synthesized by an amine coupling method via N-hydroxysuccinimide esters (NHS) at pH 8.3. (C) Antibody (Ab) modified PAR28-PEG- and PAR28-liposomes were prepared by post-insertion of PAR28-phospholipids into anionic liposomes at 4 °C for 1 h, followed by incubation with antibody at 4 °C for 1 h.
