Fig. 1.

TRIM31 was decreased both in wide type (WT) mice after middle cerebral artery occlusion (MCAO) and in primary neurons after oxygen-glucose deprivation/reperfusion (OGD/R). (A) Immunofluorescence of TRIM31 in mouse brain after 24 h MCAO. Double immunofluorescence of TRIM31 (red) and NeuN (neuron marker, green) or GFAP (astrocyte marker, green) was performed. Scale bars: 50 μm. (B) Western blot analysis of TRIM31 expression in brain tissue from WT mice after 6 h, 12 h, 24 h, 48 h and 72 h MCAO, n = 5. (C) Representative immunofluorescence image of TRIM31 in primary cultured neurons. Double immunofluorescence of TRIM31 (red) and β₃-Tubulin (neuron marker, green) was performed. Scale bars: 10 μm. (D) Western blot analysis of TRIM31 in cultured primary neurons subjected to 90 min OGD and 24 h reoxygenation (OGD/R), n = 3. Data are expressed as the mean ± SEM. *p < 0.05 for statistical analysis of the indicated groups. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)