Skip to main content
. 2021 Jun 29;45:102058. doi: 10.1016/j.redox.2021.102058

Fig. 3.

Fig. 3

TRIM31 silence enhanced the antioxidant defense and preserved mitochondria function. PC12 cells were transfected with siRNAs specifically targeting TRIM31 (siTRIM31) or its nonsense control (NC). (A) Cell viability of PC12 cells subjected to 90 min of OGD followed by 24 h of reoxygenation (OGD/R) were assayed by CCK8 assay. Knockdown of TRIM31 increased ATP production (B) and NADPH level (C) in PC12 cells after the treatment of OGD/R. (D) Representative photographs of DHE fluorescence analysis of the levels of reactive oxygen species (ROS) after the treatment of OGD/R in PC12 cells. (E) The level of ROS was analyzed by flow cytometry. (F) TRIM31 silence inhibited the downregulation of mitochondrial DNA levels induced by OGD/R. (G) Detection of JC-1 signals in PC12 cells by fluorescence microscopy. (H) TRIM31 silence upregulated the G6PD protein expression in PC12 cells subjected to OGD/R.