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Macrophage gating strategy
(A) Forward and sideward scatter gating to exclude debris.
(B) Singlet cell gating.
(C) Live cell gating.
(D) CD45+ cell gating.
(E) F4/80High population gating.
(F) CD11cHigh and CD206Low gating for M1 Macrophages, and CD11cLow and CD206Mid to High gating for M2 Macrophages.
(G) M2 Macrophages express high levels of Arginase-1.
