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Regulatory T-cells (Tregs) gating strategy
(A) Forward and sideward scatter gating to exclude debris.
(B) Singlet cell gating.
(C) Live cell gating.
(D) CD45+ cell gating.
(E) CD11b and F4/80 double-negative gating.
(F) CD11c negative gating.
(G) CD3ε+ T cell gating.
(H) CD4+ T cell and CD8+ T cell gating.
(I) CD4+FOXP3+ gating to identify Tregs
