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Monocytic tissue dendritic cells (mDCs) gating strategy
(A) Forward and sideward scatter gating to exclude debris.
(B) Singlet cell gating.
(C) Live cell gating.
(D) CD45+ cell gating.
(E) CD8αAll and Ly-6CLow gating.
(F) F4/80-CD3ε- for mDCs.
(G) CD8αLow and CD11cMid to High to identify mDCs
