Table 3. Tissue Digestion Media Recipe

Reagent	Final concentration	Amount
Dulbecco’s Modified Eagle Media (DMEM) + 10% FBS	N/A	5 mL
50 mg/mL Collagenase P	0.5 mg/mL	50 μL
1 mg/mL Deoxyribonuclease I (DNase I)	10 μg/mL	50 μL
Total	N/A	5.1 mL

Store at 4^°C for up to 1 month