Table 4.
List of antibodies used to characterize ILC2s
| Antibody | Clone | Fluorochrome | Dilution | Company and Catalog # |
|---|---|---|---|---|
| ILC2-Specific Markers | ||||
| GATA3 | L50-823 | Brilliant Violet 711 | 1/100 | BD #565449 |
| IgG1, κ Isotype Control | X40 | Brilliant Violet 711 | 1/100 | BD #563044 |
| ST2 | RMST2-2 | PerCP-eFluor710 | 1/50 | eBioscience #46-9335 |
| IgG2, κ Isotype Control | eBR2a | PerCP-eFluor710 | 1/50 | eBioscience #46-4321-80 |
| CD127 | A7R34 | Brilliant Violet 650 | 1/200 | BioLegend #135043 |
| IgG2α, κ Isotype Control | RTK2758 | Brilliant Violet 650 | 1/200 | BioLegend # 400542 |
| KLRG1 | 2F1 | Alexa Fluor 488 | 1/50 | BD #561619 |
| CD45.2 | 30-F11 | Brilliant Violet 785 | 1/2000 | BioLegend #103149 |
| ICOS | C398.4A | APC | 1/50 | eBioscience #17-9949-82 |
| Thy 1 (CD90.2) | 53-2.1 | Brilliant Violet 510 | 1/400 | BioLegend #140319 |
| CD25 | PC61 | PE-Cy7 | 1/100 | BD #561780 |
| Viability Dye | - | Near IR | 1/2000 | Invitrogen #L10119 |
| Lineage Markers | ||||
| FcεR1 | MAR-1 | PE | 1/50 | eBioscience #12-5898-83 |
| CD19 | eBioID3 | PE | 1/100 | eBioscience #12-0193-82 |
| CD3ε | 145-2C11 | PE | 1/400 | eBioscience #12-0031-85 |
| B220 | RA3-6B2 | PE | 1/200 | eBioscience #12-0452-83 |
| TCRβ | H57-597 | PE | 1/200 | eBioscience #12-5961-83 |
| TCRγ/δ | EBioGL3 | PE | 1/400 | eBioscience #12-5711-82 |
| NK1.1∗ | PK136 | PE | 1/50 | eBioscience #12-5941-82 |
| Gr-1 | RB6-8C5 | eFluor450 | 1/500 | eBioscience #48-5931-82 |
| TER-119 | TER-119 | eFluor450 | 1/50 | eBioscience #48-5921-82 |
| CD11c | N418 | eFluor450 | 1/50 | eBioscience #48-0114-82 |
| CD11b | M1/70 | eFluor450 | 1/500 | eBioscience #48-0112-82 |
Lineage markers are included as these provide a lineage-negative population to discriminate ILC2s.