Table 5.
List of antibodies used to characterize T-cells and Myeloid Cells
| Antibody | Clone | Fluorochrome | Dilution | Company and Catalog # |
|---|---|---|---|---|
| CD45.2 | 30-F11 | APC-Cy7 | 1/800 | BD #561037 |
| CD3ε | 145-2C11 | Brilliant Violet 650 | 1/12.5 | BD #563044 |
| CD8α | 53-6.7 | PerCP-Cy5.5 | 1/50 | BD #561109 |
| CD4 | GK1.5 | PE-Cy7 | 1/100 | BioLegend #100-422 |
| CD11b | M1/70 | eFluor450 | 1/2000 | eBioscience #48-0112-82 |
| CD11c | HL3 | Brilliant Violet 786 | 1/500 | BD # 563735 |
| F4/80 | BM8 | PE | 1/100 | eBioscience #12-4801-80 |
| Ly-6G/Ly-6C | RB6-8C5 | Alexa Fluor 488 | 1/2000 | eBioscience #53-5931-82 |
| Ly-6C | AL-21 | APC | 3/400 | BD #560595 |
| FOXP3 | FJK-16s | PE-eFluor610 | 1/100 | eBioscience #61-5773 |
| Arginase-1 | A1exF5 | Alexa Fluor 700 | 1/100 | eBioscience #56-3697 |
| CD206 | C068C2 | Brilliant Violet 711 | 1/200 | BioLegend #141727 |
| Viability Dye | - | Aqua | 1/250 | Invitrogen #L34965 |
(Note: Titration of antibodies required to determine an optimal dilution for your institute’s flow cytometer)