| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-GATA3 BV711 (clone L50-823) | BD Biosciences | Cat #565449 |
| Mouse monoclonal anti-IgG1,κ Isotype Control BV711 (clone X40) | BD Biosciences | Cat #563044 |
| Mouse monoclonal anti-ST2 PerCP-eFluor 710 (clone RMST2-2) | eBioscience | Cat #46-9335 |
| Mouse monoclonal anti-IgG2,κ Isotype Control PerCP-eFluor 710 (clone eBR2a) | eBioscience | Cat #46-4321-80 |
| Mouse monoclonal anti-CD127 BV650 (clone A7R34) | BioLegend | Cat #135043 |
| Mouse monoclonal anti-IgG2α,κ Isotype Control BV650 (clone RTK2758) | BioLegend | Cat # 400542 |
| Mouse monoclonal anti-KLRG1 AF488 (clone 2F1) | BD Biosciences | Cat #561619 |
| Mouse monoclonal anti-CD45.2 BV785 (clone 30-F11) | BioLegend | Cat #103149 |
| Mouse monoclonal anti-ICOS APC (clone C398.4A) | eBioscience | Cat #17-9949-82 |
| Mouse monoclonal anti-Thy 1(CD90.2) BV510 (clone 53-2.1) | BioLegend | Cat #140319 |
| Mouse monoclonal anti-CD25 PE-Cy7 (clone PC61) | BD Biosciences | Cat #561780 |
| Mouse monoclonal anti-FcεR1 PE (clone MAR-1) | eBioscience | Cat #12-5898-83 |
| Mouse monoclonal anti-CD19 PE (clone eBioID3) | eBioscience | Cat #12-0193-82 |
| Mouse monoclonal anti-CD3ε PE (clone 145-2C11) | eBioscience | Cat #12-0031-85 |
| Mouse monoclonal anti-B220 PE (Clone RA3-6B2) | eBioscience | Cat #12-0452-83 |
| Mouse monoclonal anti-TCRβ PE (clone H57-597) | eBioscience | Cat #12-5961-83 |
| Mouse monoclonal anti-TCRγ/δ PE (clone EBioGL3) | eBioscience | Cat #12-5711-82 |
| Mouse monoclonal anti-NK1.1 PE (clone PK136) | eBioscience | Cat #12-5941-82 |
| Mouse monoclonal anti-Gr-1 eF450 (clone RB6-8C5) | eBioscience | Cat #48-5931-82 |
| Mouse monoclonal anti-TER-119 eF450 (clone TER-119) | eBioscience | Cat #48-5921-82 |
| Mouse monoclonal anti-CD11c eF450 (clone N418) | eBioscience | Cat #48-0114-82 |
| Mouse monoclonal anti-CD11b eF450 (clone M1/70) | eBioscience | Cat #48-0112-82 |
| Mouse monoclonal anti-CD45.2 APC-Cy7 (clone 30-F11) | BD Biosciences | Cat #561037 |
| Mouse monoclonal anti-CD3ε BV650 (clone 145-2C11) | BD Biosciences | Cat #563044 |
| Mouse monoclonal anti-CD8α PerCP-Cy5.5 (clone 53-6.7) | BD Biosciences | Cat #561109 |
| Mouse monoclonal anti-CD4 PE-Cy7 (clone GK1.5) | BioLegend | Cat #100-422 |
| Mouse monoclonal anti-CD11c BV786 (clone HL3) | BD Biosciences | Cat #563735 |
| Mouse monoclonal anti-F4/80 PE (clone BM8) | eBioscience | Cat #12-4801-80 |
| Mouse monoclonal anti-Ly-6G/Ly-6C AF488 (clone RB6-8C5) | eBioscience | Cat #53-5931-82 |
| Mouse monoclonal anti-Ly-6C APC (clone AL-21) | Cat #560595 | |
| Mouse monoclonal anti-FOXP3 PE-eF610 (clone FJK-16s) | eBioscience | Cat #61-5773 |
| Mouse monoclonal anti-Arginase-1 AF700 (clone AlexF5) | eBioscience | Cat #56-3697 |
| Mouse monoclonal anti-CD206 BV711 (clone C068C2) | BioLegend | Cat #141727 |
| Chemicals, peptides, and recombinant proteins | ||
| Isofluorane USP | Fresenius Kabi | Cat #CP0406V2 |
| 4-Hydroxytamoxifen (4-HT) | Millipore Sigma | Cat #H6278 |
| 1× Dulbecco’s phosphate-buffered saline (DPBS) | Wisent Bioproducts | Cat #311-425-CL |
| Dulbecco’s modified Eagle media (DMEM) | Wisent Bioproducts | Cat #319-005-CL |
| Collagenase P | Roche | Cat #11213865001 |
| Deoxyribonuclease I (DNase I) | Sigma-Aldrich | Cat #D5025-15KU |
| Fetal bovine serum | Wisent Bioproducts | Cat #080-150 |
| LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit | Invitrogen | Cat #L10119 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit | Invitrogen | Cat #L34965 |
| OneComp eBeadsTM Compensation Beads | Invitrogen | Cat ##01-1111 |
| UltraComp eBeadsTM Compensation Beads | Invitrogen | Cat #01-2222 |
| FOXP3 / Transcription Factor Staining Buffer | eBioscience | Cat #00-5523-00 |
| Mouse FcR Blocking Reagent | Miltenyi Biotec | Cat #130-092-575 |
| Experimental models: Organisms/strains | ||
| Male C57BL/6 mouse with the BRafV600E, Tyr::CreER and Ptenlox4-5 genotype (6 to 8 weeks old) | Dankort D. et al., 2009 | Gift from Dr Dankort |
| Software and algorithms | ||
| FlowJo™ v10.7 | Becton Dickinson | SCR_008520 |
| Other | ||
| Depilatory cream | Nair | Cat # 6170022261 |
| Electric shaver | Wahl | Cat # 1590-1300 |
| Cotton gauze | AMD Ritmed | Cat # A3007 |
| Cotton swab | Medicom | Cat # 807 |
| P2 micropipette | Gilson | Cat # F144801 |
| 2 μL Pipette tips | SARSTEDT | Cat # 70.113 |
| 15 mL Tubes | Falcon | Cat # 352096 |
| 50 mL Tubes | Falcon | Cat # 352070 |
| 100 mm Culture dishes | Ultident | Cat # 229621 |
| 6-Well plate | Corning | Cat # 353046 |
| Cell strainer 70 μM | Falcon | Cat # 352350 |
| Cell strainer 50 μM | Sysmex Celltrics | Cat # 04-0042-2317 |
| 5 mL Syringe | BD Biosciences | Cat # 309646 |
| Pipette-Aid | Mandel | Cat # TM-155000 |
| 25 mL Pipette | SARSTEDT | Cat # 86.1685.001 |
| 5 mL Round-bottom tube | Falcon | Cat # 352008 |
| U-bottom 96-well plate | SARSTEDT | Cat # 83.3925 |
| BD LSR Fortessa | BD Biosciences | N/A |
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