Table 1.

Experimental design for high-voltage, pulsed electric field parameter optimization for PA-O1: lux disinfection in burn wounds

Group No.	Voltage, V	Pulse duration, μs	Number of pulses	Current, Aa	μC/s	C/treatment duration
1 Blank control: uninfected wounds	0	0	0	0	0	0
2 Burn injury with infection, w/o PEF (negative control)	0	0	0	0	0	0
3	500	200	80	3.1	0.116	0.050
4	500	200	100	3.1	0.093	0.062
5	500	200	120	3.1	0.078	0.074
6	500	200	160	3.1	0.058	0.099
7	500	200	180	3.1	0.052	0.112
8	500	100	200	3.1	0.047	0.062
9	700	100	30	4.3	0.430	0.013
10	700	100	40	4.3	0.323	0.017
11	700	100	50	4.3	0.258	0.022

^aEnergy was calculated based on the measurements of the current described in the Ref.,³⁵ where the same setup was used. Frequency of pulse repetition: 3 Hz.

PEF, pulsed electric field.