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. 2021 Jun 23;12:699278. doi: 10.3389/fgene.2021.699278

Figure 4.

Figure 4

Effect of the mutation on COL1A1 function. (A) Conservation analysis of this abnormal variation using Polyphen-2. (B) The 3D structure of mutated COL1A1 was different from that of the wild-type (WT) predicted structure using I-TASSER. (C) Subcellular localization of COL1A1 in HEK293 cells. The mutant COL1A1 was localized in the cytoplasm similar to the WT protein. (D) The mRNA and protein expression levels of COL1A1 in HEK293 cells. Mutant COL1A1 mRNA expression was no different than that of the WT in HEK293 cells, but the mutant COL1A1 protein expression was higher than that of the WT (P < 0.05). (E,F) Analysis of the mRNA and protein expression of COL1A1 and odontogenic differentiation markers (DSPP and OCN) during the osteogenic differentiation of hDPSCs. Values are represented as means ± SD of three independent experiments (*P < 0.05 and **P < 0.01). (G) Representative images from the ARS staining of DPSCs-MUT and DPSCs-CON at the indicated time points after differentiation induction.