Fig. 1.

Loss of TRIM21 has a protective effect on doxorubicin-induced cardiotoxicity in mice.TRIM21^+/+ or TRIM21^−/− male mice (10 to 12 week-old) were treated with doxorubicin (20 mg/kg, i.p.). (a) Kaplan–Meier survival curves were generated. Log rank test was used to compare the difference in survival between TRIM21+/+ (n=16) and TRIM21-/- (n=14), showing significant difference with p-value < 0.0001. (b) Mice were injected with saline or doxorubicin (20 mg/kg, i.p.). 5 days later, mice were photographed. Representative pictures are shown. Note doxorubicin-treated WT mice displayed smaller body weight and hair loss. (c) 5 days after doxorubicin injection, mice were subjected to echocardiography. (d) M-mode images from nine cardiac cycles for each animal were used to calculate ventricular measurements: ejection fraction, fractional shortening, heart rate, and LVIDd (left ventricular internal diameter at end-diastole). Individual data points and the mean ± standard deviation are shown. Student's t-test was used to compare the results. n.s., non-significant; **p<0.01; ***p<0.001; ****p<0.0001. (e) Abdominal photographs of mice after 5-days after doxorubicin injection. Abdominal ascites are indicated by red arrows. (f) Hearts from each group were photographed. Representative photos are shown. (g) Mice were weighted at the time of doxorubicin injection and 5 days after, relative body weight (normalized to body weight at the injection) and the heart/body weight ratio was measured. Each individual data point represents a mouse. Individual data points and the mean ± standard deviation are shown. Student's t-test was used to compare the results. n.s., non-significant; **p<0.01.