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. 2021 Jul 4;69:103456. doi: 10.1016/j.ebiom.2021.103456

Fig. 3.

Fig 3

TRIM21 negatively regulates p62-Keap1 aggregation and anti-ferroptotic effect in heart tissue.TRIM21+/+ or TRIM21−/− mice (n=5 in each group) were i.p. injected with saline or doxorubicin (20 mg/kg). Heart tissues were harvested 5 days later. (a) Heart tissues were lysed in RIPA buffer containing 1% SDS and probed for indicated proteins. (b) IHC staining was performed for indicated antibodies. IHC intensity was determined and quantified by Image J. Data shown are mean ± standard deviation. Student's t-test was used to compare the results. n.s., non-significant; ***p<0.001, ****p<0.0001. (c) Heart tissues were lysed and separated into detergent (1% Triton X-100)-soluble ‘‘S’’ and insoluble ‘‘I’’ fractions and subjected to IB. The ratio of insoluble/soluble fractions was normalized to the corresponding tubulin band and is shown on the bottom. Densitometry of immunoblot bands was determined by the Odyssey Infrared Imaging System. Data shown are mean ± standard deviation. Student's t-test was used to compare the results. n.s., non-significant; ***p<0.001, ****p<0.0001.