Skip to main content
. 2021 Apr 21;12(2):653–664. doi: 10.1016/j.jcmgh.2021.04.009

Figure 6.

Figure 6

Deficiency of caspase-11–inhibited LPS-induced hepatocytic pyroptosis and damage in vitro. (A, B) Primary hepatocytes from WT mice were cultured and treated with 1 μg/mL LPS for 6 hours. The protein level of pro-caspase-11 and cleaved caspase-11 was determined by Western blot. n = 4. (C–G) Primary hepatocytes from Casp11+/+ (WT) or Casp 11–/– mice were cultured and exposed to 1 μg/mL LPS for 6 hours. (C, D) The protein level of pro-Gsdmd and Gsdmd-N was detected by Western blot, and (E) the protein level of IL-1β in medium was detected by enzyme-linked immunosorbent assay. The level of (F) ALT and (G) AST in medium was detected. n = 4. PBS, phosphate-buffered saline. ∗∗P < .01, ∗∗∗P < .001. ns, no significance.