Figure 7.

Caspase-11 was upregulated by NF-κB signaling both in vitro and in vivo. (A–C) Primary hepatocytes from WT mice were cultured and treated with 1 μg/mL LPS and 20 μM NF-κB inhibitor JSH-23 for 6 hours. The (A) mRNA and (B, C) protein level of caspase-11 were determined by RT-qPCR and Western blot, respectively. n = 4. (D–F) WT mice were fed with MCD or regular diet for 4 weeks with the treatment of 3 mg/kg/d JSH-23 or vehicle by gavage. The (D) mRNA and (E, F) protein level of caspase-11 were determined by reverse-transcription quantitative PCR and Western blot, respectively. n = 8. ∗P < .05, ∗∗P < .01, ∗∗∗∗P < .0001.