Skip to main content
. 2021 Apr 21;12(2):653–664. doi: 10.1016/j.jcmgh.2021.04.009

Figure 8.

Figure 8

Overexpression of caspase-11 was sufficient to increase the severity of MCD-induced steatohepatitis in mice. (A) Representative scheme of the structure of the AAV9-Caspase-11 vector. (B–G) Alb-Cre mice were intravenously injected with control or caspase-11–overexpressing AAV9 and fed with MCD for 4 weeks to induce NASH or fed with regular diet as control. Paraffin-embedded liver sections were stained with (B) hematoxylin and eosin, and (C) hepatic steatosis and (D) ballooning scores were calculated. (E) Serum ALT level, (F) serum AST level, and (G) liver triglycerides were detected. n = 5 for 2 control groups, n = 8 for 2 MCD groups. (H–L) Alb-Cre mice were intravenously injected with control or caspase-11–overexpressing AAV9 and fed with MCD with the treatment of 100 mg/kg/d caspase-1 inhibitor belnacasan or vehicle by gavage for 4 weeks. Paraffin-embedded liver sections were stained with hematoxylin and eosin, and (H) hepatic steatosis and (I) ballooning scores were calculated. (J) Serum ALT level, (K) serum AST level, and (L) liver triglycerides were detected. n = 8 for 4 groups. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001. ns, no significance.