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. 2021 Feb 27;29(7):2268–2280. doi: 10.1016/j.ymthe.2021.02.025

Figure 2.

Figure 2

circFoxO1 regulates choroidal EC function in vitro

(A) RF/6A cells were transfected with scrambled (Scr) siRNA, circFoxO1 siRNA, pcDNA 3.1 vector, pcDNA 3.1-circFoxO1 (circFoxO1 OE), or were left untreated (Ctrl) for 48 h. qRT-PCR assays were conducted to detect the levels of circFoxO1 (n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). (B–E) RF/6A cells were transfected with Scr siRNA, circFoxO1 siRNA, or were left untreated (Ctrl) for 48 h. Cell viability was detected using an MTT assay (B, n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). Cell proliferation was detected using an EdU detection kit to analyze the incorporation of EdU during DNA synthesis (C, n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). Scale bar, 20 μm. Cell migration was determined using the transwell assay, and the cells that migrated through the transwell were quantified (D, n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). Scale bar, 20 μm. RF/6A cells were seeded on the Matrigel matrix. The tube-like structures were observed 6 h after cell seeding. The average length of tube formation for each field was statistically analyzed (E, n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). Scale bar, 100 μm.