Skip to main content
. 2021 Feb 27;29(7):2268–2280. doi: 10.1016/j.ymthe.2021.02.025

Figure 3.

Figure 3

circFoxO1 regulates choroidal vascular dysfunction in vivo and ex vivo

(A) C57BL/6 mice (males, 8 weeks old) received an intravitreal injection of Scr shRNA, circFoxO1 shRNA1–3, or were left untreated (Ctrl) for 2 weeks. qRT-PCR assays were conducted to detect the levels of circFoxO1 and FoxO1 mRNA in choroid (n = 6; ∗p < 0.05 versus Ctrl group, Kruskal-Wallis test, Bonferroni test). (B) C57BL/6 mice received an intravitreal injection of Scr shRNA, circFoxO1 shRNA1, or were left untreated (Ctrl). Two weeks after laser injury, IB4 labeling was conducted to label the neovascular area in flat-mounted choroidal tissues. White circles denote the neovascular area (n = 6; ∗p < 0.05 versus Ctrl group, Kruskal-Wallis test, Bonferroni test). Scale bar, 100 μm. (C) Choroidal sprouting assays were conducted to determine the angiogenic potency of choroidal explants. CD31 staining was conducted to label choroidal sprouting. Representative images of choroidal sprouting and quantification results are shown (n = 6; ∗p < 0.05 versus Ctrl group; #p < 0.05 FD+Scr shRNA group versus FD+circFoxO1 shRNA1 group; Kruskal-Wallis test, Bonferroni test). Scale bar, 500 μm.