Figure 6.

The circFoxO1/miR-145/VEGFA or ANGPT2 axis regulates choroidal EC function in vitro

(A) RF/6A cells were treated as shown. Cell viability was detected by an MTT assay (n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). (B) Cell proliferation was detected using EdU detection kits to analyze the incorporation of EdU during DNA synthesis (n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). A representative image along with the quantification result is shown. Scale bar, 20 μm. (C) Transwell assay and quantification analysis was conducted to determine the migration of RF/6A cells (n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). Representative images along with the quantification results are shown. Scale bar, 20 μm. (D) RF/6A cells were seeded on the Matrigel matrix. The tube-like structures were observed at 6 h after cell seeding. The average length of tube formation for each field was statistically analyzed (n = 4; ∗p < 0.05 versus Ctrl group, one-way ANOVA, Bonferroni test). Scale bar, 100 μm. ∗p < 0.05 versus Ctrl group; ^#p < 0.05 indicates significant difference between the marked group.